Human D-LDH(D-Lactate Dehydrogenase) ELISA Kit

Human

DLD, LDHD, Probable D-lactate dehydrogenase,mitochondrial,

Q86WU2

Sandwich

100ng/mL

0.94 ng/mL

1.57-100ng/mL

3.5H

Tissue homogenate and Cell lysate

Developmental science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-LDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to D-LDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D-LDH in the samples is then determined by comparing the OD of the samples to the standard curve.