Human VEGF-C(Vascular Endothelial Growth Factor C) ELISA Kit

Human

VEGFC, Flt4-L, VRP

P49767

Sandwich

7000pg/mL

65.63 pg/mL

109.38-7000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Cytokine;Infection immunity;Immune molecule;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGF-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VEGF-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGF-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VEGF-C in the samples is then determined by comparing the OD of the samples to the standard curve.