Rat TAF1A(Tata Box Binding Protein Associated Factor 1A) ELISA Kit

Rat

Transcription initiation factor SL1/TIF-IB subunit A, TBP-associated factor 1A, TATA box-binding protein-associated factor RNA polymerase I subunit A

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Enzyme & Kinase;Neuro science;Hormone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TAF1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TAF1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TAF1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TAF1A in the samples is then determined by comparing the OD of the samples to the standard curve.