Rat PFK-A(6-phosphofructokinase Type A) ELISA Kit

Rat

PFK-M, ATP-PFK, ATP-dependent 6-phosphofructokinase, muscle type, Phosphofructo-1-kinase isozyme A, Phosphohexokinase

Sandwich

100ng/mL

0.94 ng/mL

1.57-100ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Cytokine;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PFK-A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PFK-A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PFK-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PFK-A in the samples is then determined by comparing the OD of the samples to the standard curve.