Mouse ANG-R-Tie1(Angiopoietin Receptor Tie1) ELISA Kit

Mouse

Tyrosine kinase with immunoglobulin-like and EGF-like domains 1,TIE, JTK14,Tyrosine-protein kinase receptor Tie-1,TIE1

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;Tumor immunity;Gastroenterology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANG-R-Tie1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ANG-R-Tie1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ANG-R-Tie1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ANG-R-Tie1 in the samples is then determined by comparing the OD of the samples to the standard curve.