Human COL17(Collagen Type ⅩⅦ) ELISA Kit

Human

COL17A1, BA16H23.2, BP18, BPA-2, BPAG2, LAD-1, ERED, collagen type XVII alpha 1, collagen type XVII alpha 1 chain

Q9UMD9(COL17A1)

Sandwich

200ng/mL

1.88 ng/mL

3.13-200ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Neuro science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL17. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to COL17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of COL17 in the samples is then determined by comparing the OD of the samples to the standard curve.