Human P-GP(Permeability Glycoprotein) ELISA Kit

Human

Multidrug resistance protein 1, ATP-binding cassette sub-family B member 1, P-glycoprotein 1, CD243

P08183

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-GP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to P-GP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain P-GP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of P-GP in the samples is then determined by comparing the OD of the samples to the standard curve.