Human PIAS1(Protein Inhibitor of Activated STAT 1) ELISA Kit

Human

E3 SUMO-protein ligase PIAS1,DDXBP1, GBP, GU/RH-II, ZMIZ3,DEAD/H box-binding protein 1,E3 SUMO-protein transferase PIAS1,Gu-binding protein,Protein inhibitor of activated STAT protein 1,RNA helicase II-binding protein

O75925

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PIAS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIAS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PIAS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIAS1 in the samples is then determined by comparing the OD of the samples to the standard curve.