Human pERK1/2(Phospho Extracellular Signal Regulated Kinase 1/2) ELISA Kit

Human

MAPK1, ERK, ERK-2, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p4, p41, p41mapk, p42-MAPK, mitogen-activated protein kinase 1

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to pERK1/2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to pERK1/2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain pERK1/2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of pERK1/2 in the samples is then determined by comparing the OD of the samples to the standard curve.