Rat SP-A1(Pulmonary surfactant-associated protein A1) ELISA Kit

Rat

MGC133365, PSAP, PSPA, SFTP1, SFTPA1

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Tissue homogenate, Cell lysate, Cell culture supernatant, Alveolar lavage fluid

Endocrinology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SP-A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SP-A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SP-A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SP-A1 in the samples is then determined by comparing the OD of the samples to the standard curve.